C57BL/6 thymocytes were stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82), Anti-Mouse CD8 FITC (Product # 11-0081-82), Anti-Human/Mouse CD44 PE-eFluor® 610 (Product # 61-0441-82), Anti-Mouse CD25 PerCP-Cyanine5-5 (Product # 45-0251-82), and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) or Anti-Human/Mouse EVA1 PE. Isotype control (blue histogram) or EVA1 (purple histogram) staining on DN3 cells (CD4-CD8-CD44-CD25+) are shown and compared to EVA1 staining on DN1 cells (CD4-CD8-CD44+CD25-) (orange histogram). Viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.
Supplier Page from Thermo Fisher Scientific for EVA1 Antibody PE